msc growth supplement (Lonza)

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Lonza msc growth supplement
Msc Growth Supplement, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msc+growth+supplement/msc+growth+supplement/pm37296569-96-25-28
Average 90 stars, based on 1 article reviews

msc growth supplement - by Bioz Stars, 2026-10

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Article Title: Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs
Article Snippet: Human MSCs were obtained commercially from Lonza Corp. MSC cultures were maintained with MSC basal medium with MSC growth supplement, L -glutamine, and GA-1000 in an incubator at 37 °C.

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Article Title: Application of Human Epineural Conduit Supported with Human Mesenchymal Stem Cells as a Novel Therapy for Enhancement of Nerve Gap Regeneration
Article Snippet: In this experimental study, we used a total of 24 male athymic homozygous nude rats ( Crl:NIH- Foxn1 rnu , Charles River Laboratories, USA) weighing between 150 and 250 g. Animals were housed in pairs in hooded cages at room temperature, on a light-dark schedule of 14/10, with no limitation of food or water. .. Mesenchymal stem cells (MSC, Lonza, Inc., Switzerland) were cultured in MSC growth media supplemented with MSC growth supplement, L-glutamine, and Gentamicin-Amphotericin-B (Lonza, Inc., Switzerland) as previously reported [ ]. .. Cultured MSC (60–70% confluence) were harvested between passages 5–8 using a standard procedure with 0.25% trypsin-EDTA (Gibco-Thermo Fischer, USA) [ ].

Article Title: Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide.
Article Snippet: Lonza (Walkersville, MD, USA) was the source of hMSCs used in all of the experiments, with cells between passages 3 and 5 being utilized. .. The hMSCs were regularly cultured at 37 ◦C under 5% CO2 in a humidified atmosphere using an MSC basal medium (BM, Lonza), supplemented with 10% MSC growth supplement (Lonza); 2% L-glutamine, 0.1% GA1000; and 1% antibiotic-antimycotic solution (Sigma-Aldrich) that contained 10,000 units of penicillin, 10 mg of streptomycin, and 25 μg of amphotericin B per mL. ..

Article Title: Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide
Article Snippet: Lonza (Walkersville, MD, USA) was the source of hMSCs used in all of the experiments, with cells between passages 3 and 5 being utilized. .. The hMSCs were regularly cultured at 37 °C under 5% CO 2 in a humidified atmosphere using an MSC basal medium (BM, Lonza), supplemented with 10% MSC growth supplement (Lonza); 2% L-glutamine, 0.1% GA-1000; and 1% antibiotic-antimycotic solution (Sigma-Aldrich) that contained 10,000 units of penicillin, 10 mg of streptomycin, and 25 μg of amphotericin B per mL. ..

Article Title: 3D bioprinting of human mesenchymal stem cells-laden hydrogels incorporating MXene for spontaneous osteodifferentiation
Article Snippet: Human mesenchymal stem cells (hMSC) were purchased by Lonza (Walkersville, MD), and all experiments were conducted using hMSCs between passages 3 and 5. .. To maintain an undifferentiation state, hMSCs were routinely cultured in an MSC basal medium (Lonza) containing 10% of MSC growth supplement (Lonza), 2% l -glutamine, 0.1% GA-1000, and 25 μg of amphotericin B per mL, Sigma-Aldrich Co.) at 37 °C, 5% CO 2 in an incubator. .. For osteogenic differentiation assay, hMSCs were cultured in α-Minimum Essential Medium (basal medium) supplemented with 10 −8 M dexamethasones (Abcam, Cambridge, UK), 0.2 mM ascorbic acid (Sigma-Aldrich Co), and 10 mM β-glycerophosphate (Sigma-Aldrich Co).

Article Title: Dental Implants with Electrochemical Nanopattern Formation to Increase Osseointegration.
Article Snippet: Page 4/22 2.3 in vitro cellular assay hMSCs were purchased from Lonza (Walkersville, MD, USA), and all experiments were conducted using hMSCs between passages 3 and 5. .. For maintenance of undifferentiated state, hMSCs were cultured in MSC basal medium (Lonza) containing 10% of MSC growth supplement (Lonza), 2% of l-glutamine, 0.1% of GA-1000, and 1% of antibiotic-antimycotic solution (10,000 units of penicillin, 10 mg of streptomycin, and 25 μg of amphotericin B per mL, Sigma-Aldrich, St. Louis, MO, USA) under 5% CO2-containing 37°C humidi ed incubator. ..

Article Title: Dental implants with electrochemical nanopattern formation to increase osseointegration
Article Snippet: The hMSCs were purchased from Lonza (Walkersville, MD, USA), and all the experiments were conducted using hMSCs between passages 3 and 5. .. To maintain an undifferentiated state, the hMSCs were cultured in an MSC basal medium (Lonza) containing 10 % MSC growth supplement (Lonza), 2 % L-glutamine, 0.1 % GA-1000, and 1 % antibiotic–antimycotic solution (10 000units of penicillin, 10 mg of streptomycin, and 25 lg of amphotericin B per mL; Sigma-Aldrich, St. Louis, MO, USA) in a humidified incubator containing 5 % CO2 at 37 C. The culture media were replaced every 48 hours and the cells were sub-cultured at 80 % confluency. .. To induce osteogenic differentiation, the hMSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10–8 M dexamethasone (Abcam, Cambridge, UK), 0.2 mM ascorbic acid (Sigma-Aldrich), and 10 mM b-glycerophosphate (SigmaAldrich) containing a 1 % antibiotic–antimycotic solution.

Article Title: Reduced graphene oxide coating enhances osteogenic differentiation of human mesenchymal stem cells on Ti surfaces
Article Snippet: hMSCs were purchased from Lonza (Walkersville, MD, USA), and all experiments were conducted using hMSCs between passages 3 and 5. .. To maintain undifferentiated state, hMSCs were routinely cultured in MSC basal medium (Lonza) containing 10% of MSC growth supplement (Lonza), 2% of l-glutamine, 0.1% of GA-1000, and 1% of antibiotic-antimycotic solution (10,000 units of penicillin, 10 mg of streptomycin, and 25 μg of amphotericin B per mL, Sigma-Aldrich) at 37 °C under 5% CO 2 in a humidified atmosphere. .. For osteogenic differentiation assay, hMSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 − 8 M dexamethasone (Abcam), 0.2 mM ascorbic acid (Sigma-Aldrich), and 10 mM β-glycerolphosphate (Sigma-Aldrich).

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